BAIT

SNAPC2

PTFDELTA, SNAP45
small nuclear RNA activating complex, polypeptide 2, 45kDa
GO Process (4)
GO Function (1)
GO Component (3)
Homo sapiens
PREY

SNAPC1

PTFgamma, SNAP43
small nuclear RNA activating complex, polypeptide 1, 43kDa
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein.

Yoon JB, Roeder RG

The proximal sequence element (PSE)-binding transcription factor (PTF) specifically recognizes the PSEs of both RNA polymerase II- and RNA polymerase III-transcribed small nuclear RNA (snRNA) genes. We previously have shown that PTF purified from human HeLa cells is a multisubunit complex of four polypeptides designated PTF alpha, -beta, -gamma, and -delta. We now report the isolation and expression of cDNAs ... [more]

Mol. Cell. Biol. Jan. 01, 1996; 16(1);1-9 [Pubmed: 8524284]

Throughput

  • Low Throughput

Additional Notes

  • figure 6.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNAPC1 SNAPC2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
1192766
SNAPC1 SNAPC2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2222526
SNAPC1 SNAPC2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3037329

Curated By

  • BioGRID