NFE2L2
Gene Ontology Biological Process
- cellular response to fluid shear stress [ISO, ISS]
- cellular response to hydrogen peroxide [ISO]
- cellular response to laminar fluid shear stress [ISO]
- cellular response to oxidative stress [ISO]
- cellular response to tumor necrosis factor [ISO]
- endoplasmic reticulum unfolded protein response [ISO]
- negative regulation of cell death [IMP]
- negative regulation of endothelial cell apoptotic process [ISO]
- negative regulation of hydrogen peroxide-induced cell death [ISO]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [ISO]
- positive regulation of blood coagulation [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to stress [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- proteasomal ubiquitin-independent protein catabolic process [ISO, ISS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO, ISS]
- protein ubiquitination [ISO, ISS]
- regulation of embryonic development [ISO]
- regulation of removal of superoxide radicals [ISO]
- regulation of transcription, DNA-templated [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription cofactor binding [IPI]
- transcription regulatory region sequence-specific DNA binding [IDA]
- DNA binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription cofactor binding [IPI]
- transcription regulatory region sequence-specific DNA binding [IDA]
CHD6
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The carboxy-terminal Neh3 domain of Nrf2 is required for transcriptional activation.
Nrf2 is a transcription factor critical for the maintenance of cellular redox homeostasis. We have previously found that Nrf2 is a labile protein, and its activation in cells under stress involves mechanisms leading to its stabilization. As a modular protein, Nrf2 possesses distinct transactivation and DNA binding domains essential for its transcriptional activity. In this study, we found that the ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 8.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NFE2L2 CHD6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 810915 |
Curated By
- BioGRID