An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin.

Roghanian A, Jones DC, Pattisapu JV, Wolfe J, Young NT, Behnam B

Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-Î³1), was identified ... [more]

Cell. Immunol. Aug. 28, 2010; 265(2);120-6 [Pubmed: 20797700]

Throughput

Low Throughput

Additional Notes

table 2.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TSGA10 VIM	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Roghanian A (2010)	Low	-	BioGRID	810992
VIM TSGA10	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Roghanian A (2010)	Low	-	BioGRID	810995

Curated By

BioGRID

Interaction Summary

TSGA10

Gene Ontology Biological Process

VIM

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]glycoprotein binding [IPI]identical protein binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]scaffold protein binding [IPI]structural constituent of cytoskeleton [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS