BAIT

KT1

AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AT2G26650
potassium channel AKT1
Arabidopsis thaliana (Columbia)
PREY

CBL5

F3D13.2, F3D13_2, calcineurin B-like protein 5, AT4G01420
calcineurin B-like protein 5
GO Process (4)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Calcineurin B-like protein CBL10 directly interacts with AKT1 and modulates K(+) homeostasis in Arabidopsis.

Ren XL, Qi GN, Feng HQ, Zhao S, Zhao SS, Wang Y, Wu WH

Potassium transporters and channels play crucial roles in K(+) uptake and translocation in plant cells, which are essential for plant growth and development. AKT1 has been reported as an important K(+) channel in Arabidopsis roots involved in K(+) uptake. It is known that AKT1 is activated by a protein kinase CIPK23 interacting with two calcineurin B-like proteins CBL1/CBL9. The present ... [more]

Plant J. Jan. 20, 2013; 0(0); [Pubmed: 23331977]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KT1 CBL5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID