BAIT

POLI

RAD30B, RAD3OB
polymerase (DNA directed) iota
UPS Project
GO Process (0)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

POLH

RAD30, RAD30A, XP-V, XPV, RP11-22I24.1
polymerase (DNA directed), eta
UPS Project
GO Process (4)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

Ubiquitin mediates the physical and functional interaction between human DNA polymerases η and ι

McIntyre J, Vidal AE, McLenigan MP, Bomar MG, Curti E, McDonald JP, Plosky BS, Ohashi E, Woodgate R

Human DNA polymerases η and ι are best characterized for their ability to facilitate translesion DNA synthesis (TLS). Both polymerases (pols) co-localize in 'replication factories' in vivo after cells are exposed to ultraviolet light and this co-localization is mediated through a physical interaction between the two TLS pols. We have mapped the polη-ι interacting region to their respective ubiquitin-binding domains ... [more]

Nucleic Acids Res. Dec. 16, 2012; 0(0); [Pubmed: 23248005]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POLI POLH
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
POLI POLH
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
-
POLI POLH
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
POLI POLH
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID