PAT1
Gene Ontology Biological Process
- CENP-A containing chromatin organization [IMP]
- chromosome segregation [IMP]
- cytoplasmic mRNA processing body assembly [IGI, IMP]
- deadenylation-dependent decapping of nuclear-transcribed mRNA [IMP, IPI]
- formation of translation preinitiation complex [IMP]
- negative regulation of translational initiation [IDA, IMP]
- regulation of translational initiation [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RPL20B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
Global analysis of yeast mRNPs.
Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified 120 proteins that cross-link to mRNA, including 66 new mRNA-binding proteins. These include kinases, RNA-modification enzymes, metabolic enzymes and tRNA- and ... [more]
Throughput
- High Throughput
Additional Notes
- UV cross-linking followed by immunoprecipitation
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPL20B PAT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2437 | BioGRID | 2187097 |
Curated By
- BioGRID