POU5F1
Gene Ontology Biological Process
- BMP signaling pathway involved in heart induction [IMP]
- anatomical structure morphogenesis [TAS]
- blastocyst development [ISS]
- cardiac cell fate determination [IDA]
- cell fate commitment involved in formation of primary germ layer [IMP]
- endodermal cell fate specification [IDA]
- mRNA transcription from RNA polymerase II promoter [ISS]
- negative regulation of gene silencing by miRNA [IMP]
- positive regulation of SMAD protein import into nucleus [IDA]
- positive regulation of catenin import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of asymmetric cell division [ISS]
- regulation of gene expression [IMP]
- regulation of heart induction by regulation of canonical Wnt signaling pathway [IMP]
- regulation of methylation-dependent chromatin silencing [IDA]
- regulation of transcription, DNA-templated [IDA]
- response to wounding [IEP]
- somatic stem cell maintenance [IDA, IMP]
- transcription from RNA polymerase II promoter [ISS]
Gene Ontology Molecular Function- DNA binding [IDA]
- miRNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- miRNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
FOXD3
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The embryonic stem cell transcription factors Oct-4 and FoxD3 interact to regulate endodermal-specific promoter expression.
The POU homeodomain protein Oct-4 and the Forkhead Box protein FoxD3 (previously Genesis) are transcriptional regulators expressed in embryonic stem cells. Down-regulation of Oct-4 during gastrulation is essential for proper endoderm development. After gastrulation, FoxD3 is generally down-regulated during early endoderm formation, although it specifically remains expressed in the embryonic neural crest. In these studies, we have found that Oct-4 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FOXD3 POU5F1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID