BAIT
PSEN1
Ad3h, PS-1, PS1, S182
presenilin 1
GO Process (86)
GO Function (7)
GO Component (35)
Gene Ontology Biological Process
- Cajal-Retzius cell differentiation [IMP]
- L-glutamate transport [IMP]
- Notch receptor processing [IMP]
- Notch signaling pathway [IGI, IMP]
- T cell activation involved in immune response [IGI]
- T cell receptor signaling pathway [IGI]
- activation of MAPKK activity [IMP]
- amyloid precursor protein catabolic process [IMP]
- anagen [IGI]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- beta-amyloid formation [IMP]
- beta-amyloid metabolic process [IMP]
- blood vessel development [IMP]
- brain development [IMP]
- brain morphogenesis [IGI]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IBA]
- canonical Wnt signaling pathway [IBA]
- cell fate specification [IGI]
- cellular calcium ion homeostasis [IMP]
- cellular protein metabolic process [IDA, IGI, IMP]
- cellular response to DNA damage stimulus [IMP]
- cerebral cortex cell migration [IMP]
- cerebral cortex development [IMP]
- choline transport [IMP]
- dorsal/ventral neural tube patterning [IGI]
- embryo development [IGI, IMP]
- embryonic limb morphogenesis [IGI]
- endoplasmic reticulum calcium ion homeostasis [IGI, IMP, ISO]
- epithelial cell proliferation [IGI]
- forebrain development [IGI]
- heart development [IMP]
- heart looping [IGI]
- hematopoietic progenitor cell differentiation [IGI]
- learning or memory [IGI]
- locomotion [IGI]
- membrane protein ectodomain proteolysis [IBA, ISO]
- memory [IGI, IMP]
- mitochondrial transport [IMP]
- myeloid dendritic cell differentiation [IMP]
- myeloid leukocyte differentiation [IGI]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of apoptotic signaling pathway [IGI]
- negative regulation of axonogenesis [IMP]
- negative regulation of epidermal growth factor-activated receptor activity [IMP]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of protein kinase activity [IMP]
- negative regulation of protein phosphorylation [IGI]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of ubiquitin-protein transferase activity [IMP]
- neurogenesis [IMP]
- neuron apoptotic process [IGI]
- neuron development [IMP]
- neuron differentiation [IMP]
- neuron migration [IMP]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of catalytic activity [ISO]
- positive regulation of coagulation [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of receptor recycling [IMP]
- post-embryonic development [IMP]
- protein glycosylation [IMP]
- protein maturation [IGI]
- protein processing [IMP, ISO]
- protein transport [IGI]
- proteolysis [IGI]
- regulation of epidermal growth factor-activated receptor activity [IGI]
- regulation of phosphorylation [ISO]
- regulation of protein binding [IGI]
- regulation of resting membrane potential [IMP]
- regulation of synaptic plasticity [IGI, IMP]
- regulation of synaptic transmission, glutamatergic [IMP]
- response to oxidative stress [IGI, IMP]
- segmentation [IMP]
- single organismal cell-cell adhesion [ISO]
- skeletal system morphogenesis [IMP]
- skin morphogenesis [IMP]
- smooth endoplasmic reticulum calcium ion homeostasis [IGI]
- somitogenesis [IGI, IMP]
- synaptic vesicle targeting [IMP]
- thymus development [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA, ISO]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IDA]
- cell [IGI]
- cell cortex [IBA]
- cell surface [IBA, ISO]
- centrosome [ISO]
- ciliary rootlet [IDA]
- cytoplasmic vesicle [IDA]
- dendrite [IDA]
- dendritic shaft [IDA]
- endoplasmic reticulum [IDA, IMP, ISO]
- gamma-secretase complex [ISO]
- growth cone [IDA]
- integral component of plasma membrane [IBA, ISO]
- intracellular [IDA, IMP]
- kinetochore [ISO]
- lysosomal membrane [IBA, ISO]
- membrane [IDA]
- membrane raft [IBA, ISO]
- membrane-bounded organelle [IDA]
- mitochondrial inner membrane [IBA, ISO]
- mitochondrion [ISO]
- neuromuscular junction [IBA, ISO]
- neuron projection [ISO]
- neuronal cell body [IDA]
- nuclear membrane [ISO]
- nuclear outer membrane [ISO]
- nucleus [IDA]
- perinuclear region of cytoplasm [IBA]
- plasma membrane [IDA]
- protein complex [ISO]
- rough endoplasmic reticulum [ISO]
- smooth endoplasmic reticulum [IGI, ISO]
Mus musculus
PREY
NOTCH1
9930111A19Rik, Mis6, N1, Tan1, lin-12, RP23-306D20.12-001
notch 1
GO Process (135)
GO Function (9)
GO Component (11)
Gene Ontology Biological Process
- Notch signaling involved in heart development [IGI, IMP, ISO]
- Notch signaling pathway [IC, IDA, IMP, ISO]
- Notch signaling pathway involved in regulation of secondary heart field cardioblast proliferation [IMP]
- anagen [IMP]
- aortic valve morphogenesis [ISO]
- apoptotic process involved in embryonic digit morphogenesis [IMP]
- arterial endothelial cell differentiation [IMP]
- astrocyte differentiation [ISO]
- atrioventricular node development [IMP]
- atrioventricular valve morphogenesis [IMP]
- auditory receptor cell fate commitment [IMP]
- axonogenesis [IDA, IMP]
- branching morphogenesis of an epithelial tube [IMP]
- cardiac atrium morphogenesis [IMP]
- cardiac chamber formation [IMP]
- cardiac epithelial to mesenchymal transition [IMP]
- cardiac left ventricle morphogenesis [IMP]
- cardiac muscle cell proliferation [IMP]
- cardiac muscle tissue morphogenesis [IMP]
- cardiac right atrium morphogenesis [IMP]
- cardiac right ventricle formation [IMP]
- cardiac septum morphogenesis [IMP]
- cardiac vascular smooth muscle cell development [IMP]
- cardiac ventricle morphogenesis [IMP]
- cell differentiation [IMP]
- cell fate commitment [IMP]
- cell fate specification [IMP]
- cell migration involved in endocardial cushion formation [IMP]
- cellular response to follicle-stimulating hormone stimulus [ISO]
- cellular response to vascular endothelial growth factor stimulus [ISO]
- compartment pattern specification [IMP]
- coronary artery morphogenesis [IMP]
- coronary vein morphogenesis [IMP]
- determination of left/right symmetry [IMP]
- embryonic hindlimb morphogenesis [IMP]
- embryonic limb morphogenesis [IGI]
- endocardial cell differentiation [IMP]
- endocardial cushion morphogenesis [IMP]
- endocardium development [IMP]
- endocardium morphogenesis [IMP]
- endoderm development [IMP]
- epidermis development [IGI]
- epithelial to mesenchymal transition [IMP]
- epithelial to mesenchymal transition involved in endocardial cushion formation [IMP]
- forebrain development [IGI, IMP]
- foregut morphogenesis [IMP]
- glial cell differentiation [IMP]
- growth involved in heart morphogenesis [IMP]
- hair follicle morphogenesis [IMP]
- heart development [IMP, ISO]
- heart looping [IGI]
- heart trabecula morphogenesis [IMP]
- humoral immune response [IGI]
- in utero embryonic development [IMP]
- inflammatory response to antigenic stimulus [IGI]
- interleukin-4 secretion [IGI]
- keratinocyte differentiation [IMP]
- left/right axis specification [IGI]
- liver development [IMP]
- lung development [IMP]
- mesenchymal cell development [IMP]
- mitral valve formation [ISO]
- negative regulation of BMP signaling pathway [IMP]
- negative regulation of anoikis [ISO]
- negative regulation of calcium ion-dependent exocytosis [IMP]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of catalytic activity [IDA]
- negative regulation of cell death [IMP]
- negative regulation of cell differentiation [IMP]
- negative regulation of cell migration involved in sprouting angiogenesis [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cell-substrate adhesion [ISO]
- negative regulation of endothelial cell chemotaxis [ISO]
- negative regulation of glial cell proliferation [IDA]
- negative regulation of myoblast differentiation [ISO]
- negative regulation of myotube differentiation [IDA]
- negative regulation of neurogenesis [IDA]
- negative regulation of neuron differentiation [IGI, IMP, ISO]
- negative regulation of oligodendrocyte differentiation [IDA]
- negative regulation of ossification [IMP]
- negative regulation of osteoblast differentiation [IMP]
- negative regulation of photoreceptor cell differentiation [IMP]
- negative regulation of pro-B cell differentiation [IMP]
- negative regulation of stem cell differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- neural tube development [IMP]
- neuron differentiation [IMP]
- neuron fate commitment [IGI]
- osteoblast fate commitment [NAS]
- pericardium morphogenesis [IMP]
- positive regulation of BMP signaling pathway [IDA]
- positive regulation of JAK-STAT cascade [IDA]
- positive regulation of apoptotic process [IDA, IGI]
- positive regulation of astrocyte differentiation [IDA]
- positive regulation of cardiac muscle cell proliferation [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of endothelial cell differentiation [ISO]
- positive regulation of ephrin receptor signaling pathway [IC]
- positive regulation of epithelial cell proliferation [IGI]
- positive regulation of epithelial to mesenchymal transition [IMP, ISO]
- positive regulation of glial cell differentiation [ISO]
- positive regulation of keratinocyte differentiation [IDA, IGI]
- positive regulation of neuroblast proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter in response to hypoxia [IDA]
- positive regulation of transcription of Notch receptor target [IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- prostate gland epithelium morphogenesis [IMP]
- pulmonary valve morphogenesis [ISO]
- regulation of cardioblast proliferation [ISO]
- regulation of cell adhesion involved in heart morphogenesis [IC]
- regulation of cell migration [IMP]
- regulation of cell proliferation [ISO]
- regulation of epithelial cell proliferation [IMP]
- regulation of epithelial cell proliferation involved in prostate gland development [IMP]
- regulation of extracellular matrix assembly [IMP]
- regulation of gene expression [IMP]
- regulation of neurogenesis [IMP]
- regulation of somitogenesis [IMP]
- regulation of transcription from RNA polymerase II promoter [IGI]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [IMP]
- response to muramyl dipeptide [IDA]
- secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development [IMP]
- skeletal muscle cell differentiation [IMP]
- somatic stem cell division [IDA, IGI]
- spermatogenesis [ISO]
- sprouting angiogenesis [IMP]
- transcription from RNA polymerase II promoter [IDA]
- tube formation [ISO]
- vasculogenesis involved in coronary vascular morphogenesis [IMP]
- venous endothelial cell differentiation [IMP]
- ventricular septum morphogenesis [ISO]
- ventricular trabecula myocardium morphogenesis [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- enzyme inhibitor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- enzyme inhibitor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Murine notch homologs (N1-4) undergo presenilin-dependent proteolysis.
Oncogenic forms of Notch1, Notch2, and Notch4 appear to mimic signaling intermediates of Notch1 and suggest that the role of proteolysis in Notch signaling has been conserved. Here we demonstrate that extracellularly truncated Notch homologs are substrates for a presenilin-dependent gamma-secretase activity. Despite minimal conservation within the transmembrane domain, the requirement for a specific amino acid (P1' valine) and its ... [more]
J. Biol. Chem. Oct. 26, 2001; 276(43);40268-73 [Pubmed: 11518718]
Throughput
- Low Throughput
Curated By
- BioGRID