IRS1
Gene Ontology Biological Process
- cellular response to insulin stimulus [ISO]
- insulin receptor signaling pathway [IDA, ISO]
- insulin secretion [ISO]
- insulin-like growth factor receptor signaling pathway [ISO, ISS]
- lipid catabolic process [ISO]
- mammary gland development [ISO]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of insulin secretion [IMP, ISO]
- negative regulation of somatostatin secretion [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [IMP]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [IMP]
- positive regulation of mesenchymal cell proliferation [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [IDA, ISO]
- positive regulation of phosphorylation [IDA]
- protein heterooligomerization [IDA]
- protein kinase B signaling [ISO]
- protein localization to nucleus [ISO]
- regulation of gene expression [ISO]
- response to caffeine [IDA]
- response to insulin [ISO]
- response to peptide hormone [IDA]
Gene Ontology Molecular Function- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
Gene Ontology Cellular Component
PIK3R1
Gene Ontology Biological Process
- B cell differentiation [ISO]
- NFAT protein import into nucleus [ISO]
- aging [IEP]
- cellular glucose homeostasis [ISO, ISS]
- cellular response to UV [ISO]
- cellular response to fatty acid [IEP]
- cellular response to insulin stimulus [IEP, ISO, ISS]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- glucose metabolic process [IMP]
- growth hormone receptor signaling pathway [ISO]
- insulin receptor signaling pathway [IMP, ISS]
- insulin-like growth factor receptor signaling pathway [IMP, ISO, ISS]
- intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of blood pressure [IMP]
- negative regulation of cell-cell adhesion [IMP]
- negative regulation of cell-matrix adhesion [ISO]
- negative regulation of heart rate [IMP]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of osteoclast differentiation [ISO]
- negative regulation of proteolysis [IMP]
- negative regulation of smooth muscle cell proliferation [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- phosphatidylinositol phosphorylation [ISS]
- phosphatidylinositol-3-phosphate biosynthetic process [IDA, TAS]
- positive regulation of RNA splicing [ISO, ISS]
- positive regulation of cell migration [IMP, ISO]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO, ISS]
- positive regulation of gene expression [IMP]
- positive regulation of myoblast differentiation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription factor import into nucleus [ISO, ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor production [ISO]
- protein phosphorylation [ISO]
- protein stabilization [ISO, ISS]
- regulation of phosphatidylinositol 3-kinase activity [IDA]
- response to amino acid [IEP]
- response to cAMP [IDA]
- response to dexamethasone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO, ISS]
- response to estradiol [IEP]
- response to ethanol [IEP]
- response to fatty acid [IEP]
- response to fructose [IEP]
- response to glucocorticoid [IDA]
- response to growth factor [IEP]
- response to insulin [IDA]
- response to iron(II) ion [IEP]
- response to nutrient [IEP]
- response to progesterone [IEP]
- response to testosterone [IEP]
- response to yeast [IEP]
Gene Ontology Molecular Function- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The cross-talk between angiotensin and insulin differentially affects phosphatidylinositol 3-kinase- and mitogen-activated protein kinase-mediated signaling in rat heart: implications for insulin resistance.
Insulin and angiotensin II (AngII) may act through overlapping intracellular pathways to promote cardiac myocyte growth. In this report insulin and AngII signaling, through the phosphatidylinositol 3-kinase (PI 3-kinase) and MAPK pathways, were compared in cardiac tissues of control and obese Zucker rats. AngII induced Janus kinase 2 tyrosine phosphorylation and coimmunoprecipitation with insulin receptor substrate 1 (IRS-1) and IRS-2 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRS1 PIK3R1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PIK3R1 IRS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| IRS1 PIK3R1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PIK3R1 IRS1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID