Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HectD1 E3 Ligase Modifies Adenomatous Polyposis Coli (APC) with Polyubiquitin to Promote the APC-Axin Interaction.

Tran H, Bustos D, Yeh R, Rubinfeld B, Lam C, Shriver S, Zilberleyb I, Lee MW, Phu L, Sarkar AA, Zohn IE, Wertz IE, Kirkpatrick DS, Polakis P

The adenomatous polyposis coli (APC) protein function as a negative regulator of the Wnt signaling pathway. In this capacity, APC forms a 'destruction complex' with Axin, CK1α and GSK3β to foster phosphorylation of the Wnt effector β-catenin earmarking it for K48-linked polyubiquitylation and proteasomal degradation. APC is conjugated with K63-linked ubiquitin chains when it is bound to Axin, but it ... [more]

J. Biol. Chem. Dec. 31, 2012; 0(0); [Pubmed: 23277359]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ZRANB1 SLMAP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
ZRANB1 SLMAP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High14.92BioGRID
423455

Curated By

  • BioGRID