BAIT

UBC

HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

NDC1

NET3, TMEM48, RP4-654H19.1
NDC1 transmembrane nucleoporin
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes?

Nathan JA, Tae Kim H, Ting L, Gygi SP, Goldberg AL

Although cellular proteins conjugated to K48-linked Ub chains are targeted to proteasomes, proteins conjugated to K63-ubiquitin chains are directed to lysosomes. However, pure 26S proteasomes bind and degrade K48- and K63-ubiquitinated substrates similarly. Therefore, we investigated why K63-ubiquitinated proteins are not degraded by proteasomes. We show that mammalian cells contain soluble factors that selectively bind to K63 chains and inhibit ... [more]

EMBO J. Jan. 11, 2013; 0(0); [Pubmed: 23314748]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction
  • binds K48-linked poly-ubiquitin chains in vitro
  • identified by MS

Curated By

  • BioGRID