BAIT

PARK2

PRKN
Parkinson disease (autosomal recessive, juvenile) 2, parkin
GO Process (48)
GO Function (21)
GO Component (21)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

HDAC6

Hd6, Hdac5, Sfc6, mHDA2, RP23-198C2.1
histone deacetylase 6
GO Process (34)
GO Function (15)
GO Component (19)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6).

Hebron ML, Lonskaya I, Sharpe K, Weerasinghe PP, Algarzae NK, Shekonyan AR, Moussa CE

The importance of E3 ubiquitin ligases, involved in degradation of misfolded proteins or promotion of protein-protein interaction is increasingly recognized in neurodegeneration. TDP-43 is a predominantly nuclear protein, which regulates the transcription of thousands of genes and binds to mRNA of the E3 ubiquitin ligase parkin to regulate its expression. Wild type and mutated TDP-43 are detected in ubiquitinated forms ... [more]

J. Biol. Chem. Dec. 20, 2012; 0(0); [Pubmed: 23258539]

Throughput

  • Low Throughput

Curated By

  • BioGRID