BAIT

RGS18

regulator of G-protein signaling 18
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

GNAI3

AI158965, AW537698, Galphai3, Gnai-3, RP23-89M15.4
guanine nucleotide binding protein (G protein), alpha inhibiting 3
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A novel regulator of G-protein signaling bearing GAP activity for Galphai and Galphaq in megakaryocytes.

Nagata Y, Oda M, Nakata H, Shozaki Y, Kozasa T, Todokoro K

The regulator of G-protein signaling (RGS) negatively regulates the alpha subunit of G proteins by accelerating their intrinsic guanosine triphosphatase (GTPase) activity. Here are reported the isolation and characterization of a novel mouse RGS, termed RGS18, which is a new member of RGS subfamily B. Northern blot analysis showed that RGS18 messenger RNA was detected predominantly in spleen and hematopoietic ... [more]

Blood May. 15, 2001; 97(10);3051-60 [Pubmed: 11342430]

Throughput

  • Low Throughput

Curated By

  • BioGRID