An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hepatitis C virus RNA replication is regulated by FKBP8 and Hsp90.

Okamoto T, Nishimura Y, Ichimura T, Suzuki K, Miyamura T, Suzuki T, Moriishi K, Matsuura Y

Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a component of viral replicase and is well known to modulate the functions of several host proteins. Here, we show that NS5A specifically interacts with FKBP8, a member of the FK506-binding protein family, but not with other homologous immunophilins. Three sets of tetratricopeptide repeats in FKBP8 are responsible for interactions with ... [more]

EMBO J. Oct. 18, 2006; 25(20);5015-25 [Pubmed: 17024179]

Throughput

Low Throughput

Additional Notes

figure 4.

Curated By

BioGRID

Interaction Summary

FKBP8

Gene Ontology Biological Process

Gene Ontology Molecular Function

FK506 binding [IBA]identical protein binding [IPI]peptidyl-prolyl cis-trans isomerase activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component