BAIT

DOCK10

9330153B10Rik, A630054M16Rik, Jr4, Jr5, R75174, ZIZ3, Zizimin3, mKIAA0694
dedicator of cytokinesis 10
GO Process (2)
GO Function (2)
GO Component (2)
Mus musculus
PREY

CDC42

AI747189, AU018915, RP23-246F18.2
cell division cycle 42
GO Process (50)
GO Function (6)
GO Component (12)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes.

Nishikimi A, Meller N, Uekawa N, Isobe K, Schwartz MA, Maruyama M

A novel superfamily of guanine nucleotide exchange factors for Rho GTPases includes DOCK180 and zizimin1. The zizimin subfamily includes three genes of which only zizimin1 has been cloned. We report here the cloning of zizimin2, identified in a screen for genes enriched in germinal center B cells. Zizimin2 and zizimin1 have similar primary structures and both proteins bound and activated ... [more]

FEBS Lett. Feb. 14, 2005; 579(5);1039-46 [Pubmed: 15710388]

Throughput

  • Low Throughput

Curated By

  • BioGRID