BAIT

RB1

retinoblastoma 1
GO Process (37)
GO Function (9)
GO Component (5)

Gene Ontology Biological Process

Rattus norvegicus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta.

Los AP, Vinke FP, de Widt J, Topham MK, van Blitterswijk WJ, Divecha N

The retinoblastoma protein (pRB) is a tumor suppressor and key regulator of the cell cycle. We have previously shown that pRB interacts with phosphatidylinositol-4-phosphate 5-kinases, lipid kinases that can regulate phosphatidylinositol 4,5-bisphosphate levels in the nucleus. Here, we investigated pRB binding to another lipid kinase in the phosphoinositide cycle, diacylglycerol kinase (DGK) that phosphorylates the second messenger diacylglycerol to yield ... [more]

J. Biol. Chem. Jan. 13, 2006; 281(2);858-66 [Pubmed: 16286473]

Throughput

  • Low Throughput

Curated By

  • BioGRID