CFLAR
Gene Ontology Biological Process
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IGI]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IBA]
- negative regulation of necroptotic process [IGI]
- positive regulation of peptidase activity [IDA]
- proteolysis [IBA]
Gene Ontology Molecular Function
MAP2K1
Gene Ontology Biological Process
- Golgi inheritance [ISO]
- MAPK cascade [IMP, ISO]
- activation of MAPK activity [IMP, ISO, TAS]
- cell cycle arrest [ISO]
- cell motility [IMP]
- cell proliferation [ISO]
- cellular senescence [ISO]
- keratinocyte differentiation [IMP]
- labyrinthine layer development [IMP]
- melanosome transport [ISO]
- mitotic nuclear division [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of homotypic cell-cell adhesion [ISO]
- neuron differentiation [IMP, ISO]
- neuron projection morphogenesis [ISO]
- peptidyl-tyrosine dephosphorylation [IMP]
- placenta blood vessel development [IMP]
- positive regulation of Ras GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of cell differentiation [IDA]
- positive regulation of cell migration [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of transcription elongation from RNA polymerase II promoter [ISO]
- protein heterooligomerization [ISO]
- protein phosphorylation [IMP, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
- regulation of vascular smooth muscle contraction [ISO]
- response to axon injury [ISO]
- response to glucocorticoid [ISO]
- response to oxidative stress [ISO]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- axon [ISO]
- cell cortex [ISO]
- cytoplasm [ISO]
- cytosol [ISO, TAS]
- dendrite [ISO]
- dendrite cytoplasm [ISO]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perikaryon [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
An antiapoptotic protein, c-FLIPL, directly binds to MKK7 and inhibits the JNK pathway.
Inhibition of NF-kappaB activation increases susceptibility to tumor necrosis factor (TNF)alpha-induced cell death, concurrent with caspases and prolonged c-Jun N-terminal kinase (JNK) activation, and reactive oxygen species (ROS) accumulation. However, the detailed mechanisms are unclear. Here we show that cellular FLICE-inhibitory protein (c-FLIP) is rapidly lost in NF-kappaB activation-deficient, but not wild-type fibroblasts upon TNFalpha stimulation, indicating that NF-kappaB normally ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID