SOCS1
Gene Ontology Biological Process
- JAK-STAT cascade [IDA]
- cellular response to amino acid stimulus [IDA]
- cytokine-mediated signaling pathway [IDA, IMP]
- fat cell differentiation [IMP]
- negative regulation of JAK-STAT cascade [IMP]
- negative regulation of catalytic activity [IDA]
- negative regulation of insulin receptor signaling pathway [IDA]
- negative regulation of protein kinase activity [IBA]
- negative regulation of tyrosine phosphorylation of Stat3 protein [IDA]
- regulation of JAK-STAT cascade [IMP]
- regulation of activation of JAK2 kinase activity [IMP]
- regulation of cytokine secretion [IMP]
- regulation of protein phosphorylation [IDA]
- regulation of tyrosine phosphorylation of Stat1 protein [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CUL5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.
The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (downstream of the BC box) of the SOCS box. We now ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CUL5 SOCS1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID