BAIT

ARHGEF2

AA408978, GEF, GEF-H1, GEFH1, LFP40, Lbcl1, Lfc, P40, mKIAA0651
rho/rac guanine nucleotide exchange factor (GEF) 2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Rho-specific GEF Lfc interacts with neurabin and spinophilin to regulate dendritic spine morphology.

Ryan XP, Alldritt J, Svenningsson P, Allen PB, Wu GY, Nairn AC, Greengard P

Neurabin and spinophilin are homologous protein phosphatase 1 and actin binding proteins that regulate dendritic spine function. A yeast two-hybrid analysis using the coiled-coil domain of neurabin revealed an interaction with Lfc, a Rho GEF. Lfc was highly expressed in brain, where it interacted with either neurabin or spinophilin. In neurons, Lfc was largely found in the shaft of dendrites ... [more]

Neuron Jul. 07, 2005; 47(1);85-100 [Pubmed: 15996550]

Throughput

  • Low Throughput

Curated By

  • BioGRID