ASCL1
Gene Ontology Biological Process
- Notch signaling pathway [IGI, ISO]
- adrenal chromaffin cell differentiation [IMP]
- carotid body glomus cell differentiation [IMP]
- cell differentiation [IMP]
- cell maturation [IMP]
- central nervous system neuron development [IGI, IMP]
- cerebral cortex development [IMP]
- commitment of neuronal cell to specific neuron type in forebrain [IMP]
- enteric nervous system development [TAS]
- forebrain neuron differentiation [ISO]
- generation of neurons [IMP]
- glial cell differentiation [IGI]
- lung neuroendocrine cell differentiation [IMP]
- musculoskeletal movement, spinal reflex action [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of neuron differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- nervous system development [ISO]
- neuroblast fate determination [IMP]
- neuroblast proliferation [IGI]
- neurogenesis [IDA, ISO]
- neuron development [IMP]
- neuron differentiation [IGI, IMP]
- neuron fate commitment [IGI, IMP]
- neuron fate specification [IMP]
- neuron migration [IGI, IMP]
- noradrenergic neuron development [IMP]
- noradrenergic neuron fate commitment [ISO]
- olfactory pit development [IMP]
- oligodendrocyte cell fate commitment [IMP]
- oligodendrocyte development [IMP]
- oligodendrocyte differentiation [IMP]
- parasympathetic nervous system development [TAS]
- pattern specification process [IMP]
- positive regulation of Notch signaling pathway [IMP]
- positive regulation of cell cycle [IDA]
- positive regulation of neural precursor cell proliferation [IMP]
- positive regulation of neurogenesis [IMP]
- positive regulation of neuron apoptotic process [IMP]
- positive regulation of neuron differentiation [IDA, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- regulation of Notch signaling pathway [IMP]
- regulation of cell proliferation [IDA]
- regulation of epithelial cell differentiation [IMP]
- regulation of gene expression [IMP]
- regulation of mitotic cell cycle [IMP]
- regulation of neurogenesis [IMP]
- regulation of timing of subpallium neuron differentiation [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- spinal cord association neuron differentiation [IMP]
- spinal cord oligodendrocyte cell differentiation [IGI]
- spinal cord oligodendrocyte cell fate specification [IMP]
- stomach neuroendocrine cell differentiation [IMP]
- subpallium neuron fate commitment [IMP]
- sympathetic ganglion development [IMP]
- sympathetic nervous system development [TAS]
- transcription from RNA polymerase II promoter [ISO]
- ventral spinal cord interneuron fate commitment [IMP]
- vestibular nucleus development [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- bHLH transcription factor binding [ISO]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding transcription factor activity [IDA]
- DNA binding [IDA]
- E-box binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- bHLH transcription factor binding [ISO]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding transcription factor activity [IDA]
Gene Ontology Cellular Component
TCF3
Gene Ontology Biological Process
- B cell lineage commitment [ISO]
- Peyer's patch development [IGI]
- T cell differentiation in thymus [IMP]
- cell development [IGI]
- gastrulation [IGI]
- histone H3 acetylation [IMP]
- histone H4 acetylation [IMP]
- immunoglobulin V(D)J recombination [IMP, ISO]
- lymphocyte differentiation [IMP]
- natural killer cell differentiation [IGI]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of B cell proliferation [ISO]
- positive regulation of cell cycle [ISO]
- positive regulation of gene expression [IMP, ISO]
- positive regulation of neuron differentiation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA, IGI, ISO]
- protein stabilization [IDA]
- regulation of G1/S transition of mitotic cell cycle [ISO]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA, IGI, TAS]
- response to drug [IMP]
- response to lipopolysaccharide [IMP]
- transcription from RNA polymerase II promoter [IMP]
- transcription, DNA-templated [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- E-box binding [IDA, ISO]
- PDZ domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IMP]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- bHLH transcription factor binding [ISO]
- chromatin binding [IDA]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI, ISO]
- protein homodimerization activity [IDA, ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding [IDA, IGI, ISO]
- sequence-specific DNA binding transcription factor activity [ISO, TAS]
- transcription coactivator activity [ISO]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- vitamin D response element binding [ISO]
- DNA binding [IDA, ISO]
- E-box binding [IDA, ISO]
- PDZ domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IMP]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- bHLH transcription factor binding [ISO]
- chromatin binding [IDA]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI, ISO]
- protein homodimerization activity [IDA, ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding [IDA, IGI, ISO]
- sequence-specific DNA binding transcription factor activity [ISO, TAS]
- transcription coactivator activity [ISO]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- vitamin D response element binding [ISO]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
N-twist, an evolutionarily conserved bHLH protein expressed in the developing CNS, functions as a transcriptional inhibitor.
Members of the basic helix-loop-helix (bHLH) transcription factor family play an essential role in multiple developmental processes. During neurogenesis, positive and negative regulation by bHLH proteins is essential for proper development. Here we report the identification and initial characterization of the bHLH gene, Neuronal twist (N-twist), named for its neural expression pattern and high sequence homology and physical linkage to ... [more]
Throughput
- Low Throughput
Additional Notes
- interaction at E-box DNA sequence via EMSA
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ASCL1 TCF3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| TCF3 ASCL1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| ASCL1 TCF3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| TCF3 ASCL1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| ASCL1 TCF3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID