NR3C1
Gene Ontology Biological Process
- adrenal gland development [ISO]
- aging [IEP]
- androgen metabolic process [IEP]
- brain development [IEP]
- cellular response to dexamethasone stimulus [IMP]
- cellular response to magnesium ion [IEP]
- cellular response to steroid hormone stimulus [ISO, ISS]
- chromatin remodeling [IMP]
- chromatin-mediated maintenance of transcription [IMP]
- circadian rhythm [IEP]
- glucocorticoid mediated signaling pathway [ISO]
- glucocorticoid metabolic process [ISO]
- glucocorticoid receptor signaling pathway [ISO]
- lung development [IEP]
- mammary gland duct morphogenesis [ISO]
- maternal behavior [ISO]
- muscle atrophy [IEP]
- negative regulation of glucocorticoid mediated signaling pathway [ISO]
- negative regulation of synaptic plasticity [IMP]
- negative regulation of vascular permeability [IMP]
- positive regulation of cell growth involved in cardiac muscle cell development [IMP]
- positive regulation of dendritic spine development [IMP]
- positive regulation of glucocorticoid receptor signaling pathway [IDA]
- positive regulation of glutamate secretion [IMP]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- regulation of cell proliferation [IDA]
- regulation of glucocorticoid biosynthetic process [ISO]
- regulation of gluconeogenesis [ISO]
- regulation of glucose metabolic process [IMP]
- regulation of transcription, DNA-templated [ISO]
- response to activity [IEP]
- response to arsenic-containing substance [IDA]
- response to calcium ion [IEP]
- response to corticosterone [IEP]
- response to dexamethasone [IEP]
- response to electrical stimulus [IEP]
- response to inactivity [IEP]
- response to insulin [IEP]
- response to mercury ion [IEP]
- response to radiation [IEP]
Gene Ontology Molecular Function- DNA binding [ISO]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region DNA binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [IDA]
- glucocorticoid receptor activity [ISO]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [ISO, ISS]
- heat shock protein binding [IDA]
- hormone binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI]
- protein dimerization activity [ISO, TAS]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- receptor tyrosine kinase binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IMP, ISO, ISS]
- steroid binding [IPI, ISO]
- steroid hormone binding [ISO, ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- zinc ion binding [TAS]
- DNA binding [ISO]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region DNA binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [IDA]
- glucocorticoid receptor activity [ISO]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [ISO, ISS]
- heat shock protein binding [IDA]
- hormone binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI]
- protein dimerization activity [ISO, TAS]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- receptor tyrosine kinase binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IMP, ISO, ISS]
- steroid binding [IPI, ISO]
- steroid hormone binding [ISO, ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
HSPA4
Gene Ontology Biological Process
- chaperone-mediated protein complex assembly [ISO]
- kidney development [IEP]
- negative regulation of DNA binding [IMP]
- negative regulation of apoptotic process [IEP, IMP]
- negative regulation of cell death [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of protein phosphorylation [IMP]
- positive regulation of angiogenesis [IEP]
- positive regulation of gene expression [IMP]
- positive regulation of protein binding [IDA]
- protein import into mitochondrial outer membrane [ISO]
- response to heat [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Mutations at positions 547-553 of rat glucocorticoid receptors reveal that hsp90 binding requires the presence, but not defined composition, of a seven-amino acid sequence at the amino terminus of the ligand binding domain.
Glucocorticoid receptors (GRs) must heterocomplex with hsp90 to have an open steroid binding cleft that can be accessed by steroid. We reported that a seven-amino acid sequence (547-553 of rat GR) overlapping the amino-terminal end of the ligand binding domain is required for hsp90 binding to GR. We have now conducted saturation mutagenesis of this sequence, which appears to be ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID