BAIT

PDLIM5

1110001A05Rik, AI987914, C87059, Enh, Enh1, Enh2, Enh3, LIM
PDZ and LIM domain 5
GO Process (2)
GO Function (5)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

ACTA2

0610041G09Rik, Actvs, SMalphaA, a-SMA, alphaSMA
actin, alpha 2, smooth muscle, aorta
GO Process (3)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ENH, containing PDZ and LIM domains, heart/skeletal muscle-specific protein, associates with cytoskeletal proteins through the PDZ domain.

Nakagawa N, Hoshijima M, Oyasu M, Saito N, Tanizawa K, Kuroda S

The Enigma homologue protein (ENH), containing an N-terminal PDZ domain and three C-terminal LIM domains, is a heart and skeletal muscle-specific protein that has been shown to preferentially interact with protein kinase C beta (PKCbeta) through the LIM domains (Kuroda et al., J. Biol. Chem. 271, 31029-31032, 1996). We here demonstrate that ENH is colocalized with a cytoskeletal protein alpha-actinin ... [more]

Biochem. Biophys. Res. Commun. Jun. 07, 2000; 272(2);505-12 [Pubmed: 10833443]

Throughput

  • Low Throughput

Curated By

  • BioGRID