BAIT
PDE4D
phosphodiesterase 4D, cAMP-specific
GO Process (31)
GO Function (14)
GO Component (8)
Gene Ontology Biological Process
- T cell receptor signaling pathway [ISO]
- adrenergic receptor signaling pathway [IMP]
- adrenergic receptor signaling pathway involved in positive regulation of heart rate [IC]
- aging [ISO]
- cAMP catabolic process [IDA, IMP, ISO]
- cellular protein complex assembly [IDA]
- cellular response to cAMP [ISO]
- cellular response to epinephrine stimulus [ISO]
- cellular response to lipopolysaccharide [ISO]
- establishment of endothelial barrier [IDA]
- leukocyte migration [ISO]
- lung development [IEP]
- memory [IEP, IMP]
- multicellular organism growth [ISO]
- negative regulation of heart contraction [ISO]
- negative regulation of peptidyl-serine phosphorylation [ISO]
- negative regulation of relaxation of cardiac muscle [ISO]
- neutrophil chemotaxis [ISO]
- positive regulation of interferon-gamma production [ISO]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-5 production [ISO]
- positive regulation of smooth muscle cell migration [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- regulation of G-protein coupled receptor protein signaling pathway [IDA]
- regulation of cardiac muscle cell contraction [IMP, ISO]
- regulation of heart rate [ISO]
- regulation of protein kinase A signaling [IMP]
- regulation of receptor activity [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- smooth muscle contraction [ISO]
Gene Ontology Molecular Function- 3',5'-cyclic-AMP phosphodiesterase activity [IDA, IMP, ISO]
- 3',5'-cyclic-nucleotide phosphodiesterase activity [ISO]
- ATPase binding [ISO]
- SH3 domain binding [IPI]
- beta-2 adrenergic receptor binding [IPI]
- cAMP binding [IMP, ISO]
- cyclic-nucleotide phosphodiesterase activity [IDA]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- scaffold protein binding [ISO]
- ubiquitin protein ligase binding [ISO]
- 3',5'-cyclic-AMP phosphodiesterase activity [IDA, IMP, ISO]
- 3',5'-cyclic-nucleotide phosphodiesterase activity [ISO]
- ATPase binding [ISO]
- SH3 domain binding [IPI]
- beta-2 adrenergic receptor binding [IPI]
- cAMP binding [IMP, ISO]
- cyclic-nucleotide phosphodiesterase activity [IDA]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- scaffold protein binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
LYN
JTK8, p53Lyn, p56Lyn
LYN proto-oncogene, Src family tyrosine kinase
GO Process (58)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- B cell homeostasis [ISS]
- Fc receptor mediated inhibitory signaling pathway [ISS]
- Fc receptor mediated stimulatory signaling pathway [IBA, ISS]
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- T cell costimulation [TAS]
- blood coagulation [TAS]
- cellular response to DNA damage stimulus [IDA]
- cellular response to peptide hormone stimulus [IBA]
- cellular response to retinoic acid [IMP]
- central nervous system development [IBA]
- dendritic cell differentiation [IBA, ISS]
- erythrocyte differentiation [ISS]
- immune response-regulating cell surface receptor signaling pathway [ISS, TAS]
- inflammatory response [IBA]
- innate immune response [IBA, TAS]
- leukocyte migration [TAS]
- lipopolysaccharide-mediated signaling pathway [ISS]
- negative regulation of B cell proliferation [IBA]
- negative regulation of ERK1 and ERK2 cascade [ISS]
- negative regulation of MAP kinase activity [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of immune response [TAS]
- negative regulation of intracellular signal transduction [ISS]
- negative regulation of mast cell proliferation [IBA, ISS]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of toll-like receptor 2 signaling pathway [ISS]
- negative regulation of toll-like receptor 4 signaling pathway [ISS]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA]
- platelet activation [TAS]
- platelet degranulation [IBA, ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of cellular component movement [IDA]
- positive regulation of dendritic cell apoptotic process [IBA, ISS]
- positive regulation of mast cell proliferation [IMP]
- positive regulation of neuron projection development [IMP]
- positive regulation of stress-activated protein kinase signaling cascade [IDA]
- positive regulation of tyrosine phosphorylation of STAT protein [ISS]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- regulation of B cell apoptotic process [IBA]
- regulation of B cell receptor signaling pathway [IBA, ISS]
- regulation of ERK1 and ERK2 cascade [ISS]
- regulation of cell adhesion mediated by integrin [IMP]
- regulation of cytokine production [ISS]
- regulation of erythrocyte differentiation [ISS]
- regulation of mast cell activation [ISS]
- regulation of mast cell degranulation [IBA, ISS]
- regulation of monocyte chemotaxis [IMP]
- regulation of platelet aggregation [IBA, ISS]
- regulation of protein phosphorylation [TAS]
- response to hormone [ISS]
- signal transduction [TAS]
- signal transduction by phosphorylation [TAS]
- tolerance induction to self antigen [IBA, ISS, TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The unique N-terminal domain of the cAMP phosphodiesterase PDE4D4 allows for interaction with specific SH3 domains.
Of the five PDE4D isoenzymes, only the PDE4D4 cAMP specific phosphodiesterase was able to bind to SH3 domains. Only PDE4D4 and PDE4A5, but not any other PDE4A, B, C and D isoforms expressed in rat brain, bound to src, lyn and fyn kinase SH3 domains. Purified PDE4D4 could bind to purified lyn SH3. PDE4D4 and PDE4A5 both exhibited selectivity for ... [more]
FEBS Lett. Oct. 22, 1999; 460(1);173-7 [Pubmed: 10571082]
Throughput
- Low Throughput
Curated By
- BioGRID