BAIT
PDE4D
phosphodiesterase 4D, cAMP-specific
GO Process (31)
GO Function (14)
GO Component (8)
Gene Ontology Biological Process
- T cell receptor signaling pathway [ISO]
- adrenergic receptor signaling pathway [IMP]
- adrenergic receptor signaling pathway involved in positive regulation of heart rate [IC]
- aging [ISO]
- cAMP catabolic process [IDA, IMP, ISO]
- cellular protein complex assembly [IDA]
- cellular response to cAMP [ISO]
- cellular response to epinephrine stimulus [ISO]
- cellular response to lipopolysaccharide [ISO]
- establishment of endothelial barrier [IDA]
- leukocyte migration [ISO]
- lung development [IEP]
- memory [IEP, IMP]
- multicellular organism growth [ISO]
- negative regulation of heart contraction [ISO]
- negative regulation of peptidyl-serine phosphorylation [ISO]
- negative regulation of relaxation of cardiac muscle [ISO]
- neutrophil chemotaxis [ISO]
- positive regulation of interferon-gamma production [ISO]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-5 production [ISO]
- positive regulation of smooth muscle cell migration [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- regulation of G-protein coupled receptor protein signaling pathway [IDA]
- regulation of cardiac muscle cell contraction [IMP, ISO]
- regulation of heart rate [ISO]
- regulation of protein kinase A signaling [IMP]
- regulation of receptor activity [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- smooth muscle contraction [ISO]
Gene Ontology Molecular Function- 3',5'-cyclic-AMP phosphodiesterase activity [IDA, IMP, ISO]
- 3',5'-cyclic-nucleotide phosphodiesterase activity [ISO]
- ATPase binding [ISO]
- SH3 domain binding [IPI]
- beta-2 adrenergic receptor binding [IPI]
- cAMP binding [IMP, ISO]
- cyclic-nucleotide phosphodiesterase activity [IDA]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- scaffold protein binding [ISO]
- ubiquitin protein ligase binding [ISO]
- 3',5'-cyclic-AMP phosphodiesterase activity [IDA, IMP, ISO]
- 3',5'-cyclic-nucleotide phosphodiesterase activity [ISO]
- ATPase binding [ISO]
- SH3 domain binding [IPI]
- beta-2 adrenergic receptor binding [IPI]
- cAMP binding [IMP, ISO]
- cyclic-nucleotide phosphodiesterase activity [IDA]
- drug binding [ISO]
- enzyme binding [IPI]
- ion channel binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- scaffold protein binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SRC
ASV, SRC1, c-SRC, p60-Src, RP5-823N20.1
SRC proto-oncogene, non-receptor tyrosine kinase
GO Process (50)
GO Function (17)
GO Component (10)
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- Ras protein signal transduction [TAS]
- T cell costimulation [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- bone resorption [IBA, ISS]
- cell adhesion [IBA]
- cellular response to peptide hormone stimulus [IBA]
- cellular response to progesterone stimulus [ISS]
- central nervous system development [IBA]
- epidermal growth factor receptor signaling pathway [IBA, TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [IBA, TAS]
- integrin-mediated signaling pathway [IMP]
- intracellular estrogen receptor signaling pathway [IBA]
- intracellular signal transduction [IDA]
- leukocyte migration [TAS]
- membrane organization [TAS]
- negative regulation of anoikis [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- negative regulation of focal adhesion assembly [ISS]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- negative regulation of mitochondrial depolarization [IMP]
- negative regulation of protein homooligomerization [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- osteoclast development [IBA]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA]
- platelet activation [TAS]
- platelet-derived growth factor receptor signaling pathway [IBA]
- positive regulation of integrin activation [TAS]
- positive regulation of protein kinase B signaling [IMP]
- progesterone receptor signaling pathway [IBA, ISS]
- protein autophosphorylation [IDA]
- regulation of bone resorption [TAS]
- regulation of caveolin-mediated endocytosis [IMP]
- regulation of cell cycle [IBA]
- regulation of cell proliferation [IBA]
- regulation of cell-cell adhesion [IMP]
- regulation of early endosome to late endosome transport [IMP]
- regulation of epithelial cell migration [IMP]
- regulation of podosome assembly [IBA]
- regulation of vascular permeability [TAS]
- response to interleukin-1 [IMP]
- signal complex assembly [TAS]
- signal transduction [TAS]
- stress fiber assembly [IMP]
- transforming growth factor beta receptor signaling pathway [IMP]
Gene Ontology Molecular Function- SH2 domain binding [IPI]
- SH3/SH2 adaptor activity [TAS]
- enzyme binding [IPI]
- ephrin receptor binding [IPI]
- growth factor receptor binding [IPI]
- heme binding [IDA]
- hormone receptor binding [IBA]
- integrin binding [TAS]
- ion channel binding [IPI]
- kinase activity [TAS]
- non-membrane spanning protein tyrosine kinase activity [IBA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA, TAS]
- protein tyrosine kinase activity [EXP, IDA, TAS]
- receptor binding [IPI]
- scaffold protein binding [IPI]
- SH2 domain binding [IPI]
- SH3/SH2 adaptor activity [TAS]
- enzyme binding [IPI]
- ephrin receptor binding [IPI]
- growth factor receptor binding [IPI]
- heme binding [IDA]
- hormone receptor binding [IBA]
- integrin binding [TAS]
- ion channel binding [IPI]
- kinase activity [TAS]
- non-membrane spanning protein tyrosine kinase activity [IBA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA, TAS]
- protein tyrosine kinase activity [EXP, IDA, TAS]
- receptor binding [IPI]
- scaffold protein binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The unique N-terminal domain of the cAMP phosphodiesterase PDE4D4 allows for interaction with specific SH3 domains.
Of the five PDE4D isoenzymes, only the PDE4D4 cAMP specific phosphodiesterase was able to bind to SH3 domains. Only PDE4D4 and PDE4A5, but not any other PDE4A, B, C and D isoforms expressed in rat brain, bound to src, lyn and fyn kinase SH3 domains. Purified PDE4D4 could bind to purified lyn SH3. PDE4D4 and PDE4A5 both exhibited selectivity for ... [more]
FEBS Lett. Oct. 22, 1999; 460(1);173-7 [Pubmed: 10571082]
Throughput
- Low Throughput
Curated By
- BioGRID