SYCP3
Gene Ontology Biological Process
- chiasma assembly [IDA, IGI]
- female meiosis chromosome segregation [IDA]
- female meiosis chromosome separation [NAS]
- female meiosis sister chromatid cohesion [IDA]
- lateral element assembly [IGI]
- meiotic DNA repair synthesis [IGI]
- meiotic nuclear division [IDA]
- mitotic nuclear division [IDA]
- negative regulation of apoptotic process [IMP]
- sperm chromatin condensation [IGI]
- spermatogenesis [IGI, IMP]
- spermatogenesis, exchange of chromosomal proteins [ISO]
- synapsis [IMP]
- synaptonemal complex assembly [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SYCP2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis.
During meiosis, the arrangement of homologous chromosomes is tightly regulated by the synaptonemal complex (SC). Each SC consists of two axial/lateral elements (AEs/LEs), and numerous transverse filaments. SC protein 2 (SYCP2) and SYCP3 are integral components of AEs/LEs in mammals. We find that SYCP2 forms heterodimers with SYCP3 both in vitro and in vivo. An evolutionarily conserved coiled coil domain ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SYCP2 SYCP3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID