SNAP25
Gene Ontology Biological Process
- calcium ion-dependent exocytosis of neurotransmitter [ISO]
- endosomal transport [ISO]
- long-term memory [ISO]
- long-term synaptic potentiation [IMP]
- neurotransmitter secretion [IMP]
- positive regulation of hormone secretion [ISO]
- potassium ion transmembrane transport [ISO]
- regulation of establishment of protein localization [IMP]
- regulation of ion transmembrane transport [ISO]
- regulation of neuron projection development [ISO]
- sleep [ISO]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- synaptic vesicle priming [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [ISO]
- SNARE complex [ISO]
- actin cytoskeleton [ISO]
- axon [ISO]
- axonal growth cone [ISO]
- cell [IMP]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- endosome [ISO]
- filopodium [ISO]
- growth cone [ISO]
- intracellular organelle [ISO]
- lamellipodium [ISO]
- membrane [IDA]
- membrane raft [ISO]
- myelin sheath [IDA]
- neuron projection [ISO]
- neuronal cell body [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO, TAS]
- synapse [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [ISO]
- terminal bouton [ISO]
- trans-Golgi network [IDA]
- vesicle [ISO]
- voltage-gated potassium channel complex [ISO]
HGS
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule".
The structure of the endosomal-associated protein, Hrs, has been determined with cryo-electron microscopy. Hrs interacts with a number of proteins, including SNAP-25 and STAM1, forming a complex that binds ubiquitin moieties. Analytical ultracentrifugation studies revealed that Hrs exists as a hexamer. The symmetry and the structure of the hexameric form of Hrs were determined with the single-particle reconstruction method. Hrs ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SNAP25 HGS | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.8725 | BioGRID | 2669825 |
Curated By
- BioGRID