An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Calmodulin antagonizes a calcium-activated SCF ubiquitin E3 ligase subunit, FBXL2, to regulate surfactant homeostasis.

Chen BB, Coon TA, Glasser JR, Mallampalli RK

Calmodulin is a universal calcium-sensing protein that has pleiotropic effects. Here we show that calmodulin inhibits a new SCF (Skp1-Cullin-F-box) E3 ligase component, FBXL2. During Pseudomonas aeruginosa infection, SCF (FBXL2) targets the key enzyme, CCTÎ±, for its monoubiquitination and degradation, thereby reducing synthesis of the indispensable membrane and surfactant component, phosphatidylcholine. P. aeruginosa triggers calcium influx and calcium-dependent activation of ... [more]

Mol. Cell. Biol. May. 01, 2011; 31(9);1905-20 [Pubmed: 21343341]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FBXL2 CALM1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Chen BB (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FBXL2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatidylinositol 3-kinase regulatory subunit binding [ISO]protein binding [IPI]protein phosphatase binding [ISO]ubiquitin-protein transferase activity [IMP, ISO]

Gene Ontology Cellular Component

CALM1