BAIT
COL5A1
AI413331, RP23-475B13.1
collagen, type V, alpha 1
GO Process (14)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- blood vessel development [IMP]
- cell adhesion [ISO]
- cell migration [ISO]
- collagen biosynthetic process [ISO]
- collagen fibril organization [IGI, IMP, ISO]
- extracellular fibril organization [ISO]
- eye morphogenesis [ISO]
- heart morphogenesis [IGI]
- integrin biosynthetic process [ISO]
- negative regulation of endodermal cell differentiation [ISO]
- regulation of cellular component organization [IMP]
- skin development [IMP, ISO]
- tendon development [IGI, IMP]
- wound healing, spreading of epidermal cells [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
SMAD4
AW743858, D18Wsu70e, DPC4, Madh4
SMAD family member 4
GO Process (54)
GO Function (23)
GO Component (10)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- SMAD protein complex assembly [ISO]
- SMAD protein signal transduction [ISO]
- anterior/posterior pattern specification [IMP]
- atrioventricular canal development [IGI]
- atrioventricular valve formation [IGI]
- axon guidance [IMP]
- brainstem development [IMP]
- branching involved in ureteric bud morphogenesis [IGI]
- cardiac septum development [IGI]
- cell proliferation [IMP]
- developmental growth [IMP]
- endocardial cell differentiation [IMP]
- endoderm development [IMP]
- endothelial cell activation [IMP]
- epithelial to mesenchymal transition involved in endocardial cushion formation [IMP]
- formation of anatomical boundary [IMP]
- gastrulation [IMP]
- gastrulation with mouth forming second [IMP]
- in utero embryonic development [IMP]
- intracellular signal transduction [ISO]
- kidney development [IMP]
- mesoderm development [IMP]
- metanephric mesenchyme morphogenesis [IMP]
- negative regulation of cell death [IMP]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IGI]
- negative regulation of protein catabolic process [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- nephrogenic mesenchyme morphogenesis [IMP]
- neural crest cell differentiation [IMP]
- neuron fate commitment [IMP]
- palate development [IMP]
- positive regulation of BMP signaling pathway [ISO]
- positive regulation of SMAD protein import into nucleus [IMP]
- positive regulation of cell proliferation involved in heart valve morphogenesis [IMP]
- positive regulation of epithelial to mesenchymal transition [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of transforming growth factor beta receptor signaling pathway [ISO]
- regulation of binding [IDA]
- regulation of cell proliferation [IGI, IMP]
- regulation of hair follicle development [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [ISO]
- regulation of transforming growth factor beta2 production [ISO]
- response to hypoxia [ISO]
- response to transforming growth factor beta [ISO]
- sebaceous gland development [IMP]
- somite rostral/caudal axis specification [IMP]
- tissue morphogenesis [IMP]
- transcription from RNA polymerase II promoter [ISO]
- transforming growth factor beta receptor signaling pathway [IGI, ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- I-SMAD binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [IPI]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- chromatin binding [IDA]
- collagen binding [IPI]
- core promoter proximal region sequence-specific DNA binding [ISO]
- filamin binding [ISO]
- identical protein binding [ISO]
- protein binding [IPI]
- protein binding transcription factor activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [ISO]
- DNA binding [IDA, ISO]
- I-SMAD binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [IPI]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- chromatin binding [IDA]
- collagen binding [IPI]
- core promoter proximal region sequence-specific DNA binding [ISO]
- filamin binding [ISO]
- identical protein binding [ISO]
- protein binding [IPI]
- protein binding transcription factor activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of Smads with collagen types I, III, and V.
Ontogenesis of the mammalian orofacial region is controlled by numerous developmental signals, including those initiated by the transforming growth factors beta (TGFbetas). Targeted deletion of the genes encoding several of the TGFbetas in mice has been shown to result in clefts of the secondary palate. Members of the TGFbeta family of growth factors utilize intracellular Smads as signal transducers. Smads ... [more]
Biochem. Biophys. Res. Commun. Oct. 31, 2003; 310(4);1117-23 [Pubmed: 14559231]
Throughput
- Low Throughput
Curated By
- BioGRID