MPL
Gene Ontology Biological Process
Gene Ontology Cellular Component
PIK3CA
Gene Ontology Biological Process
- glucose metabolic process [IMP]
- hypomethylation of CpG island [IDA]
- negative regulation of anoikis [ISO]
- negative regulation of fibroblast apoptotic process [IDA]
- negative regulation of neuron apoptotic process [IMP]
- phosphatidylinositol phosphorylation [IBA, ISO]
- phosphatidylinositol-3-phosphate biosynthetic process [ISO]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of protein kinase activity [IDA]
- protein kinase B signaling [IMP]
- protein phosphorylation [IDA]
- regulation of genetic imprinting [IDA]
- regulation of multicellular organism growth [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The c-Mpl ligand (thrombopoietin) stimulates tyrosine phosphorylation of Jak2, Shc, and c-Mpl.
c-Mpl is a member of the cytokine receptor superfamily, expressed primarily on hematopoietic cells. Recently, the c-Mpl ligand was cloned and found to have thrombopoietic activity. In this paper we report that ligand binding induced tyrosine phosphorylation in BaF3 cells engineered to express the murine Mpl receptor (BaF3/mMpl). Phosphorylation occurred within 1 min at cytokine concentrations sufficient for proliferation of ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID