An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A Matrix Protein Silences Transposons and Repeats through Interaction with Retinoblastoma-Associated Proteins.

Xu Y, Wang Y, Stroud H, Gu X, Sun B, Gan ES, Ng KH, Jacobsen SE, He Y, Ito T

Epigenetic regulation helps to maintain genomic integrity by suppressing transposable elements (TEs) and also controls key developmental processes, such as flowering time [1-3]. To prevent TEs from causing rearrangements and mutations, TE and TE-like repetitive DNA sequences are usually methylated, whereas histones are hypoacetylated and methylated on specific residues (e.g., H3 lysine 9 dimethylation [H3K9me2]) [4, 5]. TEs and repeats ... [more]

Curr. Biol. Feb. 05, 2013; 0(0); [Pubmed: 23394836]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT2G42940 NFC5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Xu Y (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NFC5

Gene Ontology Cellular Component

AT2G42940

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]chromatin DNA binding [IDA]