A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Mutator phenotype induced by aberrant replication.

Liu VF, Bhaumik D, Wang TS

We have identified thermosensitive mutants of five Schizosaccharomyces pombe replication proteins that have a mutator phenotype at their semipermissive temperatures. Allele-specific mutants of DNA polymerase delta (poldelta) and mutants of Polalpha, two Poldelta subunits, and ligase exhibited increased rates of deletion of sequences flanked by short direct repeats. Deletion of rad2(+), which encodes a nuclease involved in processing Okazaki fragments, ... [more]

Mol. Cell. Biol. Feb. 01, 1999; 19(2);1126-35 [Pubmed: 9891047]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Additional Notes

data not shown

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RQH1 CDC23	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vo TV (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC23

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA primase activity [IDA]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

RQH1