BAIT
MAD1
coiled-coil domain-containing protein MAD1, L000000974, YGL086W
Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
GO Process (5)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
- kinetochore [IDA, IMP]
- nuclear pore [IDA]
- nucleus [IDA]
Saccharomyces cerevisiae (S288c)
PREY
TIF6
CDC95, translation initiation factor 6, L000004376, YPR016C
Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
GO Process (6)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- assembly of large subunit precursor of preribosome [IMP]
- maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]
- rRNA processing [IGI]
- ribosomal large subunit biogenesis [IMP, IPI]
- ribosomal subunit export from nucleus [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Saccharomyces cerevisiae Genetics Predicts Candidate Therapeutic Genetic Interactions at the Mammalian Replication Fork.
The concept of synthetic lethality has gained popularity as a rational guide for predicting chemotherapeutic targets based on negative genetic interactions between tumor-specific somatic mutations and a second-site target gene. One hallmark of most cancers that can be exploited by chemotherapies is chromosome instability (CIN). Because chromosome replication, maintenance, and segregation represent conserved and cell-essential processes, they can be modeled ... [more]
G3 (Bethesda) Feb. 01, 2013; 3(2);273-82 [Pubmed: 23390603]
Quantitative Score
- 0.037701843 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- SGA analysis for synthetic lethal interactions between mutations whose human orthologs are found to be mutated in cancers, and the deletion mutant collection, where the interaction probability P < 0.05
Curated By
- BioGRID