BAIT

ALG3

RHK1, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase, L000002668, YBL082C
Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
Saccharomyces cerevisiae (S288c)
PREY

FLC2

FAD transporter FLC2, HUF2, YAL053W
Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A combined system to engineer glycosylation efficiency and glycan structure in Saccharomyces cerevisiae.

Nasab FP, Aebi M, Bernhard G, Frey AD

We describe a novel synthetic N-glycosylation pathway to produce recombinant proteins carrying human-like N-glycans in Saccharomyces cerevisiae addressing at the same time glycoform and glycosylation efficiency. A double mutant strain Δalg3Δalg11, in which the N-glycans are not matured to their native high mannose structure, was used. In this mutant strain lipid-linked Man(3)GlcNAc(2) is built up on the cytoplasmic side of ... [more]

Appl. Environ. Microbiol. Nov. 30, 2012; 0(0); [Pubmed: 23204425]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide modification (APO:0000131)

Additional Notes

  • flipping of glycosylated LLO's into ER luman in alg3 alg11 double mutant is partially rescued by flc2* mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FLC2 ALG3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1297BioGRID
2076310

Curated By

  • BioGRID