BAIT

ASR1

ubiquitin-protein ligase ASR1, YPR093C
Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
UPS Project
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RPC10

RPB12, DNA-directed RNA polymerase core subunit RPB12, ABC10-alpha, L000001686, YHR143W-A
RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative Proteomics Demonstrates that the RNA Polymerase II Subunits Rpb4 and Rpb7 Dissociate During Transcription Elongation.

Mosley AL, Hunter GO, Sardiu M, Smolle ME, Workman JL, Florens L, Washburn MP

Eukaryotic RNA Polymerase II is a twelve subunit enzyme that is responsible for the transcription of messenger RNA. Two of the subunits of RNA Polymerase II, Rpb4 and Rpb7, have been shown to dissociate from the enzyme under a number of specific laboratory conditions. However, a biological context for the dissociation of Rpb4 and Rpb7 has not been identified. We ... [more]

Mol. Cell Proteomics Feb. 15, 2013; 0(0); [Pubmed: 23418395]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASR1 RPC10
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
ASR1 RPC10
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
2202795

Curated By

  • BioGRID