DCUN1D1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VHL
Gene Ontology Biological Process
- cell morphogenesis [NAS]
- cellular response to hypoxia [TAS]
- negative regulation of apoptotic process [NAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [IDA]
- positive regulation of cell differentiation [NAS]
- positive regulation of transcription, DNA-templated [IMP]
- protein stabilization [NAS]
- protein ubiquitination [IDA, IMP]
- proteolysis [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of transcription, DNA-templated [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
DCNL1 FUNCTIONS AS A SUBSTRATE SENSOR AND ACTIVATOR OF CULLIN 2-RING LIGASE.
Substrate engagement by F-box proteins promotes NEDD8 modification of cullins, which is necessary for the activation of cullin-RING E3 ubiquitin ligase (CRL). However, the mechanism by which substrate recruitment triggers cullin neddylation remains unclear. Here, we identify DCNL1 as a component of CRL2 called ECV (Elongins BC/CUL2/VHL) and show that molecular suppression of DCNL1 attenuates CUL2 neddylation. DCNL1 via its ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID