BAIT

SHP1

UBX1, protein phosphatase regulator SHP1, L000002746, YBL058W
UBX (ubiquitin regulatory X) domain-containing protein; regulates Glc7p phosphatase activity; shp1 mutants are impaired in growth and mitotic progression; functions in growth and mitotic progression require Cdc48p binding; mitotic phenotype is caused by reduced Glc7p activity; interacts with ubiquitylated proteins, required for degradation of a ubiquitylated model substrate
Saccharomyces cerevisiae (S288c)
PREY

DAM1

L000004420, YGR113W
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

The budding yeast cdc48(shp1) complex promotes cell cycle progression by positive regulation of protein phosphatase 1 (glc7).

Boehm S, Buchberger A

The conserved, ubiquitin-selective AAA ATPase Cdc48 regulates numerous cellular processes including protein quality control, DNA repair and the cell cycle. Cdc48 function is tightly controlled by a multitude of cofactors mediating substrate specificity and processing. The UBX domain protein Shp1 is a bona fide substrate-recruiting cofactor of Cdc48 in the budding yeast S. cerevisiae. Even though Shp1 has been proposed ... [more]

PLoS ONE Feb. 19, 2013; 8(2);e56486 [Pubmed: 23418575]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • Over-expression of a non-phosphorylatable DAM1 allele allows shp1-7 mutants to grow better at higher temperatures
  • Over-expression of a non-phosphorylatable DAM1 allele allows the mad2 shp1-7 double mutant (synthetic lethal) to grow

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SHP1 DAM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4302BioGRID
2027449

Curated By

  • BioGRID