An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping.

Fenger-Gron M, Fillman C, Norrild B, Lykke-Andersen J

Decapping is a key step in mRNA turnover. However, the composition and regulation of the human decapping complex is poorly understood. Here, we identify three proteins that exist in complex with the decapping enzyme subunits hDcp2 and hDcp1: hEdc3, Rck/p54, and a protein in decapping we name Hedls. Hedls is important in decapping because it enhances the activity of the ... [more]

Mol. Cell Dec. 22, 2005; 20(6);905-15 [Pubmed: 16364915]

Throughput

Low Throughput

Additional Notes

figure 5.
figure 6.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UPF1 DCP2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lejeune F (2003)	Low	-	BioGRID	-
UPF1 DCP2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lykke-Andersen J (2002)	Low	-	BioGRID	-
DCP2 UPF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lykke-Andersen J (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UPF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA]RNA binding [NAS]chromatin binding [IDA]helicase activity [NAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

DCP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

exoribonuclease activity, producing 5'-phosphomonoesters [TAS]m7G(5')pppN diphosphatase activity [IDA]protein binding [IPI]