BAIT

PCBD2

2700061N24Rik, Dcoh2, Dcohm
pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 2
GO Process (4)
GO Function (2)
GO Component (2)
Mus musculus
PREY

HNF1A

AI323641, HNF1, HNF1-alpha, HNF1[a], Hnf-1, Hnf1alpha, LFB1, Tcf-1, Tcf1
HNF1 homeobox A
GO Process (34)
GO Function (14)
GO Component (8)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Biochemical and structural basis for partially redundant enzymatic and transcriptional functions of DCoH and DCoH2.

Rose RB, Pullen KE, Bayle JH, Crabtree GR, Alber T

An inherited form of diabetes, maturity-onset diabetes of the young type 3 (MODY3), results from mutations in the transcriptional activator, hepatocyte nuclear factor-1alpha (HNF1alpha). Transcription by HNF1alpha is stimulated by the bifunctional coactivator DCoH (dimerization cofactor of HNF1). Strikingly, an HNF1alpha deletion in mice causes more severe phenotypes than a DCoH deletion. It has been hypothesized that a DCoH homolog, ... [more]

Biochemistry Jun. 15, 2004; 43(23);7345-55 [Pubmed: 15182178]

Throughput

  • Low Throughput

Curated By

  • BioGRID