Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Expression and localization of Kv1 potassium channels in rat dorsal and ventral spinal roots.

Utsunomiya I, Yoshihashi E, Tanabe S, Nakatani Y, Ikejima H, Miyatake T, Hoshi K, Taguchi K

We investigated the expression and localization of Kv1 channels in dorsal spinal roots (DRs) and ventral spinal roots (VRs) in rats. Among Kv1.1-1.6 tested by RT-PCR, mRNAs of Kv1.1, 1.2, and 1.5 were moderately expressed, those of Kv1.3 and Kv1.6 were weakly expressed, and that of Kv1.4 was hardly expressed at all in both DRs and VRs, whereas all six ... [more]

Exp. Neurol. Mar. 01, 2008; 210(1);51-8 [Pubmed: 18053989]

Throughput

  • Low Throughput

Curated By

  • BioGRID