Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Development and application of a DNA microarray-based yeast two-hybrid system.

Suter B, Fontaine JF, Yildirimman R, Rasko T, Schaefer MH, Rasche A, Porras P, Vazquez-Alvarez BM, Russ J, Rau K, Foulle R, Zenkner M, Saar K, Herwig R, Andrade-Navarro MA, Wanker EE

The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]

Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]

Throughput

  • High Throughput

Additional Notes

  • wild-type N-terminal HTT fragment (HD506-Q23)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PIAS1 HTT
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
3390254
HTT PIAS1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
3390255

Curated By

  • BioGRID