Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Development and application of a DNA microarray-based yeast two-hybrid system.

Suter B, Fontaine JF, Yildirimman R, Rasko T, Schaefer MH, Rasche A, Porras P, Vazquez-Alvarez BM, Russ J, Rau K, Foulle R, Zenkner M, Saar K, Herwig R, Andrade-Navarro MA, Wanker EE

The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]

Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]

Throughput

High Throughput

Additional Notes

wild-type N-terminal HTT fragment (HD506-Q23)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HTT ZDHHC17	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Singaraja RR (2002)	Low	-	BioGRID	-
ZDHHC17 HTT	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Singaraja RR (2002)	Low	-	BioGRID	-
ZDHHC17 HTT	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Butland SL (2014)	High	-	BioGRID	-
HTT ZDHHC17	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Faber PW (1998)	Low	-	BioGRID	-
HTT ZDHHC17	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Singaraja RR (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HTT

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-tubulin binding [IDA]dynactin binding [IPI]dynein intermediate chain binding [IDA]identical protein binding [IPI]ion channel binding [IDA]p53 binding [IPI]protein binding [IPI]transcription factor binding [IBA]

Gene Ontology Cellular Component

ZDHHC17

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]magnesium ion transmembrane transporter activity [IDA]palmitoyltransferase activity [IDA]protein binding [IPI]protein-cysteine S-palmitoyltransferase activity [IDA]signal transducer activity [IMP]