BAIT
HTT
HD, IT15
huntingtin
GO Process (8)
GO Function (8)
GO Component (12)
Gene Ontology Biological Process
- Golgi organization [IMP]
- establishment of mitotic spindle orientation [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- organ development [IBA]
- positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA]
- regulation of protein phosphatase type 2A activity [IMP]
- retrograde vesicle-mediated transport, Golgi to ER [IMP]
- vesicle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HEYL
HESR3, HEY3, HRT3, bHLHb33
hes-related family bHLH transcription factor with YRPW motif-like
GO Process (15)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- Notch signaling pathway [IDA, TAS]
- atrioventricular valve morphogenesis [ISS]
- cardiac epithelial to mesenchymal transition [ISS]
- cardiac ventricle morphogenesis [ISS]
- endocardial cushion morphogenesis [ISS]
- epithelial to mesenchymal transition involved in endocardial cushion formation [ISS]
- mesenchymal cell development [ISS]
- negative regulation of androgen receptor activity [IDA]
- negative regulation of androgen receptor signaling pathway [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [ISS]
- positive regulation of neuron differentiation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- pulmonary valve morphogenesis [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- AF-1 domain binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISS]
- microsatellite binding [IDA]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [IDA]
- transcription factor binding [NAS]
- AF-1 domain binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISS]
- microsatellite binding [IDA]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [IDA]
- transcription factor binding [NAS]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Development and application of a DNA microarray-based yeast two-hybrid system.
The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]
Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]
Throughput
- High Throughput
Additional Notes
- wild-type N-terminal HTT fragment (HD506-Q23)
Curated By
- BioGRID