BAIT
ATXN1
ATX1, D6S504E, SCA1
ataxin 1
GO Process (3)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ASNS
ASNSD, TS11
asparagine synthetase (glutamine-hydrolyzing)
GO Process (10)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- asparagine biosynthetic process [IC, IDA]
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- cellular protein metabolic process [TAS]
- cellular response to glucose starvation [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- negative regulation of apoptotic process [IMP]
- positive regulation of mitotic cell cycle [IDA]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Development and application of a DNA microarray-based yeast two-hybrid system.
The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]
Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]
Throughput
- High Throughput
Additional Notes
- wild-type Ataxin (ATXN1-Q32)
Curated By
- BioGRID