PNKP
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [IDA, NAS]
- DNA repair [IGI]
- DNA-dependent DNA replication [NAS]
- dephosphorylation [IDA]
- nucleic acid phosphodiester bond hydrolysis [NAS]
- nucleotide phosphorylation [IDA]
- nucleotide-excision repair, DNA damage removal [NAS]
- polynucleotide 3' dephosphorylation [IDA]
- response to oxidative stress [IDA]
- response to radiation [NAS, TAS]
Gene Ontology Molecular Function- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
PRKDC
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IBA]
- double-strand break repair via nonhomologous end joining [TAS]
- innate immune response [TAS]
- negative regulation of protein phosphorylation [ISS]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of type I interferon production [TAS]
- regulation of circadian rhythm [ISS]
- signal transduction involved in mitotic G1 DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV.
Nonhomologous end joining (NHEJ) is the major DNA double-strand break (DSB) repair pathway in mammalian cells. A critical step in this process is DNA ligation, involving the Xrcc4-DNA ligase IV complex. DNA end processing is often a prerequisite for ligation, but the coordination of these events is poorly understood. We show that polynucleotide kinase (PNK), with its ability to process ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKDC PNKP | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 3711506 | |
| PNKP PRKDC | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID