BAIT
IRS1
G972R, IRS-1
insulin receptor substrate 1
GO Process (28)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- cellular response to insulin stimulus [IDA, ISO]
- insulin receptor signaling pathway [IBA, ISO]
- insulin secretion [IMP]
- insulin-like growth factor receptor signaling pathway [ISO]
- lipid catabolic process [IMP]
- mammary gland development [IGI]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of somatostatin secretion [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [IGI]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [ISO]
- positive regulation of mesenchymal cell proliferation [IGI]
- positive regulation of phosphatidylinositol 3-kinase activity [IMP, ISO]
- positive regulation of phosphorylation [ISO]
- protein heterooligomerization [ISO]
- protein kinase B signaling [IGI]
- protein localization to nucleus [IDA, IMP]
- regulation of gene expression [IMP]
- response to caffeine [ISO]
- response to insulin [ISO]
- response to peptide hormone [ISO]
Gene Ontology Molecular Function- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
INSR
4932439J01Rik, CD220, D630014A15Rik, IR, IR-A, IR-B
insulin receptor
GO Process (43)
GO Function (20)
GO Component (12)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- activation of MAPK activity [ISO]
- activation of protein kinase B activity [ISO]
- activation of protein kinase activity [ISO]
- cellular response to growth factor stimulus [IDA]
- cellular response to insulin stimulus [ISO]
- epidermis development [IMP]
- exocrine pancreas development [IMP]
- glucose homeostasis [ISO]
- heart morphogenesis [ISO]
- insulin receptor signaling pathway [IMP, ISO]
- intracellular signal transduction [ISO]
- male sex determination [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of transporter activity [ISO]
- organ morphogenesis [IMP]
- peptidyl-tyrosine autophosphorylation [IDA]
- peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of developmental growth [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glycogen biosynthetic process [IMP, ISO]
- positive regulation of glycolytic process [ISO]
- positive regulation of glycoprotein biosynthetic process [ISO]
- positive regulation of mitosis [IMP, ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of phosphorylation [ISO]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of respiratory burst [ISO]
- protein autophosphorylation [ISO]
- protein heterotetramerization [ISO]
- protein phosphorylation [ISO]
- regulation of embryonic development [ISO]
- regulation of hydrogen peroxide metabolic process [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to tumor necrosis factor [ISO]
- signal transduction by phosphorylation [ISO]
- transformation of host cell by virus [ISO]
Gene Ontology Molecular Function- 3-phosphoinositide-dependent protein kinase binding [ISO]
- ATP binding [ISO]
- GTP binding [ISO]
- PTB domain binding [ISO]
- insulin binding [ISO]
- insulin receptor substrate binding [ISO]
- insulin-activated receptor activity [ISO]
- insulin-like growth factor I binding [ISO]
- insulin-like growth factor II binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- lipoic acid binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase activity [ISO]
- receptor signaling protein tyrosine kinase activity [ISO]
- 3-phosphoinositide-dependent protein kinase binding [ISO]
- ATP binding [ISO]
- GTP binding [ISO]
- PTB domain binding [ISO]
- insulin binding [ISO]
- insulin receptor substrate binding [ISO]
- insulin-activated receptor activity [ISO]
- insulin-like growth factor I binding [ISO]
- insulin-like growth factor II binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- lipoic acid binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase activity [ISO]
- receptor signaling protein tyrosine kinase activity [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The TSC1-2 tumor suppressor controls insulin-PI3K signaling via regulation of IRS proteins.
Insulin-like growth factors elicit many responses through activation of phosphoinositide 3-OH kinase (PI3K). The tuberous sclerosis complex (TSC1-2) suppresses cell growth by negatively regulating a protein kinase, p70S6K (S6K1), which generally requires PI3K signals for its activation. Here, we show that TSC1-2 is required for insulin signaling to PI3K. TSC1-2 maintains insulin signaling to PI3K by restraining the activity of ... [more]
J. Cell Biol. Jul. 19, 2004; 166(2);213-23 [Pubmed: 15249583]
Throughput
- Low Throughput
Curated By
- BioGRID