MAPK6
Gene Ontology Biological Process
Gene Ontology Molecular Function
MAPKAPK2
Gene Ontology Biological Process
- 3'-UTR-mediated mRNA stabilization [ISO]
- G2 DNA damage checkpoint [ISO]
- cellular response to DNA damage stimulus [ISO]
- cellular response to vascular endothelial growth factor stimulus [ISO]
- inflammatory response [IMP]
- inner ear development [IDA]
- mRNA stabilization [IMP]
- macropinocytosis [IMP]
- peptidyl-serine phosphorylation [ISO]
- protein phosphorylation [IDA]
- regulation of interleukin-6 production [IMP]
- regulation of tumor necrosis factor production [IMP, ISO]
- response to cytokine [ISO]
- response to lipopolysaccharide [IMP]
- response to stress [ISO]
- toll-like receptor signaling pathway [IMP]
- vascular endothelial growth factor receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Scaffolding by ERK3 regulates MK5 in development.
Extracellular-regulated kinase 3 (ERK3, MAPK6) is an atypical member of the ERKs, lacking the threonine and tyrosine residues in the activation loop, carrying a unique C-terminal extension and being mainly regulated by its own protein stability and/or by autophosphorylation. Here we show that ERK3 specifically interacts with the MAPK-activated protein kinase 5 (MK5 or PRAK) in vitro and in vivo. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MAPK6 MAPKAPK2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID