BAIT

FBXW4

Dac, Fbw4, SHFM3, SHSF3, dactylin, dactylyn
F-box and WD-40 domain protein 4
GO Process (4)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Mus musculus
PREY

SKP1A

15kDa, 2610043E24Rik, 2610206H23Rik, EMC19, OCP-II, OCP2, SKP1, Tceb1l, p19A, p19Skp1, RP23-223A11.5
S-phase kinase-associated protein 1A
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10.

Maruyama S, Hatakeyama S, Nakayama K, Ishida N, Kawakami K, Nakayama K

The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, ... [more]

Genomics Dec. 01, 2001; 78(3);214-22 [Pubmed: 11735228]

Throughput

  • Low Throughput

Curated By

  • BioGRID