RAP1
Gene Ontology Biological Process
- chromatin organization involved in regulation of transcription [IDA]
- chromatin silencing at silent mating-type cassette [IGI]
- chromatin silencing at telomere [IGI]
- establishment of chromatin silencing at telomere [IPI]
- establishment of protein localization to chromatin [IPI]
- establishment of protein localization to telomere [IPI]
- negative regulation of chromatin silencing [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protection from non-homologous end joining at telomere [IMP]
- regulation of glycolytic by positive regulation of transcription from RNA polymerase II promoter [IGI]
- telomere maintenance [IMP]
- telomere maintenance via telomere lengthening [IDA, IMP]
Gene Ontology Molecular Function- DNA binding, bending [IDA]
- G-quadruplex DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- TBP-class protein binding RNA polymerase II transcription factor activity [IDA]
- TFIID-class transcription factor binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- nucleosomal DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- telomeric DNA binding [IDA]
- DNA binding, bending [IDA]
- G-quadruplex DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- TBP-class protein binding RNA polymerase II transcription factor activity [IDA]
- TFIID-class transcription factor binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- nucleosomal DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- telomeric DNA binding [IDA]
Gene Ontology Cellular Component
SMT3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1.
In eukaryotes, permanent inhibition of the non-homologous end joining (NHEJ) repair pathway at telomeres ensures that chromosome ends do not fuse. In budding yeast, binding of Rap1 to telomere repeats establishes NHEJ inhibition. Here, we show that the Uls1 protein is required for the maintenance of NHEJ inhibition at telomeres. Uls1 protein is a non-essential Swi2/Snf2-related translocase and a Small ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth in exponential phase (APO:0000310)
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- smt3-3R mutation rescues growth of rap1-DAmP uls1 double mutant
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMT3 RAP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 147995 | |
| RAP1 SMT3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2255 | BioGRID | 1949580 |
Curated By
- BioGRID