BAIT

ABRA

STARS
actin-binding Rho activating protein
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ABLIM3

HMFN1661
actin binding LIM protein family, member 3
GO Process (3)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Two novel members of the ABLIM protein family, ABLIM-2 and -3, associate with STARS and directly bind F-actin.

Barrientos T, Frank D, Kuwahara K, Bezprozvannaya S, Pipes GC, Bassel-Duby R, Richardson JA, Katus HA, Olson EN, Frey N

In addition to regulating cell motility, contractility, and cytokinesis, the actin cytoskeleton plays a critical role in the regulation of transcription and gene expression. We have previously identified a novel muscle-specific actin-binding protein, STARS (striated muscle activator of Rho signaling), which directly binds actin and stimulates serum-response factor (SRF)-dependent transcription. To further dissect the STARS/SRF pathway, we performed a yeast ... [more]

J. Biol. Chem. Mar. 16, 2007; 282(11);8393-403 [Pubmed: 17194709]

Throughput

  • Low Throughput

Curated By

  • BioGRID