BAIT

PIGF

phosphatidylinositol glycan anchor biosynthesis, class F
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

PIGO

mFLJ00350, RP23-124L1.7
phosphatidylinositol glycan anchor biosynthesis, class O
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol.

Shishioh N, Hong Y, Ohishi K, Ashida H, Maeda Y, Kinoshita T

Many eukaryotic cell surface proteins are anchored to the membrane via glycosylphosphatidylinositol (GPI). GPI is synthesized from phosphatidylinositol by stepwise reactions and attached en bloc to nascent proteins. In mammalian cells, the major GPI species transferred to proteins is termed H7. By attachment of an additional ethanolamine phosphate (EtNP) to the second mannose, H7 can be converted to H8, which ... [more]

J. Biol. Chem. Mar. 11, 2005; 280(10);9728-34 [Pubmed: 15632136]

Throughput

  • Low Throughput

Curated By

  • BioGRID